16S of enrichment culture: petroleum environment - SRA

SRX24462422: 16S of enrichment culture: petroleum environment
1 ILLUMINA (Illumina NovaSeq 6000) run: 76,410 spots, 34.3M bases, 11.5Mb downloads

Design: Following the centrifugation of 50 mL clay suspension at 5000g for XX minutes, Genomic DNA was extracted from the resulting pellet utilizing the Qiagen soil extraction kit (Qiagen, 47023, Valencia, CA). Subsequently, PCR amplification of hypervariable 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Submitted by: China University of Geosciences, Beijing

Study: The abiotic oxidation of petroleum hydrocarbons using reduced iron-bearing clay minerals affect the microbial degradation function.

PRJNA1108008 • SRP505874 • All experiments • All runs

show Abstracthide Abstract

Here we undertook a comprehensive study that involved both abiotic oxidation (by OH) and subsequent biological oxidation (by thermophiles in Tengchong hot spring) of petroleum hydrocarbons (contains a wide variety of hydrocarbons). These processes were facilitated by the oxidation and reduction of an iron-rich smectite, nontronite (NAu-2), respectively. Our objective was to assess the influence of altered petroleum hydrocarbons on their biodegradation mechanisms upon Fe cycling. We analyzed the Fe(II) concentration, hydrocarbons, oxygen-containing compounds, mineral transformation, bacterial community, and functional genes. These analyses were supported by wet-chemical method, mineralogy, organic geochemistry, molecular biology and metagenomics.

Sample: ArchaeaRN2-161D

SAMN41220766 • SRS21215370 • All experiments • All runs

Organism: Sphingomonadales bacterium

Library:

Name: Archaea-RN2-161D

Instrument: Illumina NovaSeq 6000

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 76,410 spots, 34.3M bases, 11.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR28905967	76,410	34.3M	11.5Mb	2024-06-08

ID:: 32778515

SRA

Sequence Read Archive

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